Laboratory of Calcium Binding Proteins

Head: Anna Filipek

 

Degrees:

2009 Professor of Biological Sciences, nomination by the President of the Republic of Poland, Nencki Institute of Experimental Biology, PAS
2000 DSc Habil, Nencki Institute of Experimental Biology, PAS
1990 PhD­­ in Biology, Nencki Institute of Experimental Biology, PAS
1985 MSc in Chemistry, University of Warsaw

 

Research trainings:

1990 - 1991 Post-doc, Vollum Institute, OHSU, Portland, USA
1990 Cell Biology course, Aarhus, Denmark
1989 Biophysics and Chemistry Institute, Gottingen, Germany         
1988 University Zurich-Irchel, Switzerland  

 

Professional employments:

2009-present Associate Professor and Head the of Laboratory of Calcium Binding Proteins, Nencki Institute of Experimental Biology, PAS
2002-2009 Assistant professor,  Nencki Institute of Experimental Biology, PAS
1985-2002 Assistant, Nencki Institute of Experimental Biology, PAS

 

Honors and fellowship:

2014 Jerzy Konorski Award of the Polish Neuroscience Society and the Committee of Neurological Sciences
2003 Silver Cross of Merit, Nencki Institute of Experimental Biology, PAS
2008 Gold Cross of Merit, Nencki Institute of Experimental Biology, PAS
2001 Award of the Department of Biological Sciences, PAS
1990 FEBS fellowship (2 weeks)
1989 Max Planck fellowship (3 months)
1988 FEBS fellowship (3 months)
1987 Polish Biochemical Society Award (Mozolowski)
1986 Polish Histochemistry and Cytochemistry Society Award

Staff: Agata Aniszewska, Katarzyna Bartkowska, Anastasiia Bohush (PhD student), Ruzanna Djavadian, Ewelina Jurewicz, Beata Kądziołka (PhD student), Wiesława Leśniak, Sara Rosińska (PhD student), Barbara Sobiak (PhD student), Paulina Sobolewska (PhD student), Beata Tepper (PhD student)


Research profile:

Research carried by the laboratory centers on: the role of a calcium binding protein S100A6, and its ligand, CacyBP/SIP, in signaling pathways involved in cell proliferation and differentiation; involvement and activity of MAP kinases in signaling pathways under normal and stress conditions; the role of Hsp90 and its co-chaperones, and of tau and α-synuclein in neurodegenerative diseases; transcriptional and epigenetic regulation of the S100 protein family gene expression. To study these processes we apply various biological and biochemical methods, among them are: cell transfection, immunoprecipitation, Western blot, immunocyto/histochemistry, ELISA, luciferase assay, gel-shift, chromatin immunoprecipitation (ChIP), bisulfite DNA modification. Another line of research conducted in the laboratory is investigation of the molecular mechanisms regulating development of the cerebellum in the opossum (Monodelphis domestica) and search for molecular basis of corpus callosum development during evolution. This study requires a multidisciplinary approach from behavioral observations to gene/protein expression.

 

Current research activities:

  • studies of CacyBP/SIP and MAP kinases in cytoskeletal organization, cell proliferation and differentiation
  • investigation of CacyBP/SIP gene expression
  • studies of Hsp90 co-chaperones and α-synuclein in neurodegenerative diseases
  • studies of extracellular activity of the S100 proteins
  • investigation of epigenetic factors regulating S100 genes expression during keratinocyte differentiation
  • construction of a transgenic opossum (Monodelphis domestica); development of the cerebellum; generation of callosum-like structures in the opossum
  • investigation of interleukin 6 and its receptor in brain functioning

 

Selected publications:

Sobiak B., Graczyk-Jarzynka A., Leśniak W. (2016) Comparison of DNA Methylation and Expression Pattern of S100 and Other Epidermal Differentiation Complex Genes in Differentiating Keratinocytes. J Cell Biochem, 117: 1092-1098.

 

Topolska-Woś A.M., Shell S.M,. Kilańczyk E., Szczepanowski R.H., Chazin W.J., Filipek A. (2015) Dimerization and phosphatase activity of calcyclin-binding protein/Siah-1 interacting protein: the influence of oxidative stress. FASEB J, 29: 1711–1724.

 

Aniszewska A., Chłodzińska N., Bartkowska K., Winnicka M.M., Turlejski K., Djavadian R.L. (2015) The expression of interleukin-6 and its receptor in various brain regions and their roles in exploratory behavior and stress responses. J Neuroimmunol, 284: 1-9.

 

Bartkowska K., Aniszewska A., Turlejski K., Djavadian R.L. (2014) Distribution and function of TrkB receptors in the developing brain of the opossum Monodelphis domestica. Develop Neurobiol, 74: 707-722.

 

Jurewicz E., Wyroba E., Filipek A. (2018) Tubulin-dependent secretion of S100A6 and cellular signaling pathways activated by S100A6-integrin β1 interaction. Cell Signal, 42: 21-29.