Laboratory of Electron Microscopy

Head: Elżbieta WYROBA

Staff: Henryk BILSKI, Szymon SUSKI

The Laboratory of Electron Microscopy was established in 1973 and provides access/assistance to investigators from the scientific institutions from all over the country and their cooperating partners from abroad in the usage of the following equipment:

1. High Performance Biology Transmission electron microscope JEM 1400 (JEOL Co., Japan, 2008) equipped with energy-dispersive full range X-ray microanalysis system (EDS INCA Energy TEM, Oxford Instruments, UK), tomographic holder and 11 Megapixel TEM Camera MORADA G2 (EMSIS GmbH, Germany).

The above-mentioned equipment was installed within the project sponsored by the European Union Structural Funds:
Centre of Advanced Technology BIM ’Equipment purchase for the Laboratory of Biological and Medical Imaging’.

2. JEM 1200 EX electron microscope (manufactured in 1986) with ASID scanning device.

3. Scanning electron microscope JSM S1 (purchased in 1973).

The Other equipment such as: Ultramicrotomes (LKB, Sweden), Critical point drying system (Polaron, UK), Vacuum evaporator (JEOL Co., Japan), and Cryoultramicrotome (Leica, Austria) are also available.

In 2011 over 16 000 digital electron micrographs and almost 1400 EDS spectra were collected by the investigators affiliated with 17 different scientific institutions. The continuous efforts have been undertaken to enable new methods in electron microscopy to be introduced and available to all the users such as full qualitative and quantitative X–ray microanalysis (EDS) enabling mapping the elements.

Lab provides services in the procedure of mild drying of the biological samples in liquid CO2 (critical point drying), coating of the biological samples with neutral metals and carbon evaporation of formvar-coated specimen grids for transmission electron microscopy and microanalysis. Continuous maintenance of the equipment and a lot of repairs of the vacuum, electronic and mechanical systems are performed.

Public education and dissemination activities of the Laboratory include:

• The Workshop 3D Imaging and Mapping of Elements by X-ray microanalysis in Electron Microscopy (November 7-8, 2012) supported by BIO-IMAGINE Project (EU FP7 REGPOT) with the goal to bring together a diverse group of scientists using various modern techniques of 3D imaging/reconstruction, qualitated and quantitative X-ray microanalysis and elements mapping in electron microscopy in order to present these cutting-edge methods to the researchers from all over the country and in particular to the young scientists. The Workshop consisted of a series of lectures and of practicals.
• EU event ‘Faces behind Science – Night with Researcher’ (project 044769) in 2006
• Lectures, practical training and presentations targeting undergraduate students
• Special sessions open to public entitled “Secrets of the cells” during the twelve consecutive “Festivals of Science” (1997-2015) – presenting the details of cell ultrastructure using three different electron microscopes to more than 1400 participants.

Selected publications:

 

Sobierajska K, Wieczorek K, Ciszewski WM, Sacewicz-Hofman I, Wawro ME, Wiktorska M, Boncela J, Papiewska-Pajak I, Kwasniak P, Wyroba E, Cierniewski CS, Niewiarowska J. (2016) β-III tubulin modulates the behavior of Snail overexpressed during the epithelial-to-mesenchymal transition in colon cancer cells. Biochim Biophys Acta - Molecular Cell Research 1863(9):2221-33.


Ehrlich H, M. Motylenko, P. .V. Sundareshwar, A. Ereskovsky, I. Zgłobicka, T. Noga, T. Płociński, M. V. Tsurkan, E. Wyroba, S. Suski, H. Bilski, M. Wysokowski, H. Stöcker, A. Makarova, D. Vyalikh, J. Walter, S. L. Molodtsov, V. V. Bazhenov, I. Petrenko, (2016) Multiphase Biomineralization: Enigmatic Invasive Siliceous Diatoms Produce Crystalline Calcite Advanced Functional Materials 26:2503 – 2510 + Supporting Information DOI: 10.1002/adfm.201504891, 1 – 23.

 

Kowalewska Ł, Mazur R, Suski S, Garstka M, Mostowska A. (2016) Three-Dimensional Visualization of the Tubular-Lamellar Transformation of the Internal Plastid Membrane Network during Runner Bean Chloroplast Biogenesis. Plant Cell. 28: 875-91.


Łabuz J,S.Samardakiewicz,P.Hermanowicz,E.Wyroba,M.Pilarska,H. Gabryś (2016) Blue light-dependent changes in loosely bound calcium in Arabidopsis mesophyll cells: an X-ray microanalysis study J.Exp.Botany 67(13):3953-64.

 

Hall M. H., Magalska A., Malinowska M., Ruszczycki B., Czaban I., Patel S., Ambrożek-Latecka M., Zołocińska E., Broszkiewicz H., Parobczak K., Nair R. R., Rylski M., Pawlak R., Bramham C. R., Wilczyński G. M. (2015) Localization and regulation of PML bodies in the adult mouse brain. Brain Struct Funct, DOI 10.1007/s00429-015-1053-4.

 

Płóciennikowska A., Zdioruk M. I., Traczyk G., Świątkowska A., Kwiatkowska K. (2015) LPS-induced clustering of CD14 triggers generation of PI(4,5)P2. J Cell Sci. 128:4096-4111.

 

Zajkowski T., Nieznanska H., Nieznanski K. (2015) Stabilization of microtubular cytoskeleton protects neurons from toxicity of N-terminal fragment of cytosolic prion protein. Biochimica et Biophysica Acta, 1853: 2228–2239

 

Urbanska P., Song K., Joachimiak E., Krzemien-Ojak L., Koprowski P., Hennessey T., Jerka-Dziadosz M., Fabczak H., Gaertig J., Nicastro D., Wloga D. (2015) The CSC proteins FAP61 and FAP251 build the basal substructures of radial spoke 3 in cilia. Mol. Biol. Cell.26: 1463–1475.

 

Samardakiewicz S., Krzeszowiec-Jeleń W., Bednarski W., Jankowski A., Suski S., Gabryś H., Woźny A. (2015) Pb - induced avoidance - like chloroplast movements in fronds of Lemna trisulca L. PloS One, 10, e0116757.

 

Wyroba E., Suski S., Miller K., Bartosiewicz R.(2015) Biomedical and agricultural applications of energy dispersive X-ray spectroscopy in electron microscopy. Cell Molec. Biol Lett. 20, 488–509.

 

Rabęda I., Bilski H., Mellerowicz E. J., Napieralska A., Suski S., Woźny A., Krzesłowska M. (2015) Colocalization of low-methylesterified pectins and Pb deposits in the apoplast of aspen roots exposed to lead. Environmental Pollution, 205: 315–326.

 

Wilczyński, G.M. (2014) Significance of higher-order chromatin architecture for neuronal function and dysfunction. Neuropharmacology 80:28–33.

 

Fulara A., Hernik A., Nieznańska H., Dzwolak W. (2014) Covalent defects restrict supramolecular self-assembly of homopolypeptides: case study of β2-fibrils of poly-L-glutamic acid. PLoS One 9, e105660

 

Kacprzyk-Stokowiec A., Kulma M., Traczyk G., Kwiatkowska K., Sobota A., Dadlez M. (2014) Crucial Role of Perfringolysin O D1 Domain in Orchestrating Structural Transitions Leading to Membrane-perforating Pores: a hydrogen-deuterium exchange study. J Biol Chem. 289: 28738–28752.

 

Surmacz-Chwedoruk W., Malka I., Bożycki Ł., Nieznańska H., Dzwolak W. (2014) On the Heat Stability of Amyloid-Based Biological Activity: Insights from Thermal Degradation of Insulin Fibrils. PLoS One. 9, e86230

 

Chaturvedi M., Molino Y., Sreedhar B., Khrestchatisky M., Kaczmarek L. (2014) Tissue inhibitor of matrix metalloproteinases-1 loaded poly(lactic-co-glycolic acid) nanoparticles for delivery across the blood–brain barrier. International Journal of Nanomedicine, 9: 575–588

 

Kwiatkowska K., Marszałek–Sadowska E., Traczyk G., Koprowski P., Musielak M., Ługowska A., Kulma M., Grzelczyk A., Sobota A. (2014) Visualization of cholesterol deposits in lysosomes of Niemann-Pick type C fibroblasts using recombinant perfringolysin O. Orphanet Journal of Rare Diseases 9:64.

 

Michalec K., Mysiorek C., Kuntz M., Bérézowski V., Szczepankiewicz A.A., Wilczyński G.M., Cecchelli R., Nałęcz K.A. (2014) Protein kinase C restricts transport of carnitine by amino acid transporter ATB0,+ apically localized in the blood-brain barrier. Arch Biochem Biophys. 554: 28–35.

 

Bednarczyk P., Wieckowski M.R., Broszkiewicz M., Skowronek K., Siemen D., Szewczyk A.(2013) Putative Structural and Functional Coupling of the Mitochondrial BKCa Channel to the Respiratory Chain. PloS ONE  8, e68125.

 

Boncela J., Przygodzka P., Wyroba E., Papiewska-Pajak I., Cierniewski C.S. (2013) Secretion of SerpinB2 from endothelial cells activated with inflammatory stimuli. Exp Cell Res. 319: 1213–1219.

 

Czeredy M., Samluk Ł., Michalec K., Tułodziecka K., Skowronek K., Nałęcz K.A. (2013) Caveolin-1 a novel interacting partner of organic cation/carnitine transporter (octn2): effect of protein kinase c on this interaction in rat astrocytes, PloS One, 8, e82105.

 

Bregier C., Krzemień-Ojak L., Włoga D., Jerka-Dziadosz M., Joachimiak E., Batko K., Filipiuk I., Smietanka U., Gaertig J., Fabczak S., Fabczak H. (2013)  PHLP2 is essential and plays a role in ciliogenesis and microtubule assembly in Tetrahymena thermophila. J Cell Physiol. 228: 2175–2189.

 

Jurewicz E., Ostrowska Z., Jozwiak J., Redowicz M.J., Lesniak W., Moraczewska J., Filipek A. (2013) CacyBP/SIP as a novel modulator of the thin filament. Biochimica et Biophysica Acta 1833: 761–766.

 

Ciechomska I.A., Gabrusiewicz K., Szczepankiewicz A.A., Kaminska B. (2013) Endoplasmic reticulum stress triggers autophagy in malignant glioma cells undergoing cyclosporine a-induced cell death. Oncogene, 32: 1518–1529.

 

Jerka-Dziadosz M., Koll F., Włoga D., Gogendeau D., Garreau de Loubresse N., Ruiz F., Fabczak S., Beisson J. (2013) A Centrin3-dependent, transient, appendage of the mother basal body guides the positioning of the daughter basal body in Paramecium. Protist,164: 352–368.



Publications list: wik.nencki.gov.pl