LABORATORY OF IMAGING TISSUE STRUCTURE AND FUNCTION

Head: Tytus Bernaś

 

Degrees:
2003 PhD in Biophysics, Jagiellonian University, Poland
1996 MSc in Biology, Jagiellonian University, Poland

 

Research trainings:
2008-2011 Post-doctoral research fellow (UE  FP7  Marie  Curie),The  Royal  College of Surgeons (RCSI), Dublin, Ireland
2004-2006 Post-doctoral research fellow, The Purdue University, West Lafayette, USA
1997-2001 PhD course in biophysics, Department of Biophysics, Biochemistry and Biotechnology Jagiellonian University, Krakow, Poland

 

Professional employments:
2012-present Head of the Laboratory of Imaging Tissue Structure and Function, Nencki Institute of Experimental Biology
2007-2011 Assistant professor at the Department of Biophysics, Biochemistry and Biotechnology Jagiellonian University, Krakow, Poland
2003-2008 Assistant professor at the Department of Plant Anatomy and Cytology, University of Silesia, Katowice, Poland
2002-2003 Research assistant position at the Department of Plant Anatomy and Cytology, University of Silesia, Katowice, Poland.
1996-1997 Research assistant at the Institute of Molecular Biology, Jagiellonian University, Krakow, Poland

Staff: Małgorzata Całka, Miguel Angel Lermo Jimenez (PhD student), Maciej Krupa (PhD student), Natalia Nowak, Błażej Ruszczycki, Hanna Sas-Nowosielska, Małgorzata Śliwińska, Artur Wolny

 


Research profile:

 

The Laboratory was established in 2012 as a core facility providing a spectrum of microscopic techniques dedicated to functional and structural studies of biological samples. It is equipped with optical and electron microscopes enabling application of various imaging methods including time-lapse, multi-dimensional (confocal, multiphoton, deconvolution), time-resolved  (fluorescence  lifetime  and  fluorescence  correlation  spectroscopy), multispectral and high-resolution (STORM, EM, ISM) microscopy of live and fixed material. These studies are supported by image analysis and visualization algorithms, developed by the group to derive quantitative results from obtained data. Since its foundation, the Laboratory has provided equipment and expertise to support a wide range of research projects concerning e.g. neuron architecture in physiological and pathological processes, cell motility, structure and dynamics of cell membranes and analysis of protein dynamics and interaction in living cells.

Moreover, the laboratory runs its own scientific projects, focused, mainly, on the structure and dynamics of cell nucleus in DNA replication and repair, and during cell migration.

 

Current research activities:

 

Analysis of replication factories as dynamic nuclear bodies, specialized in DNA replication. Particularly, their spatial distribution, dynamics and interactions of replication proteins, (PCNA, CAF1 and HP1beta).

• Validation of new fluorescent probes for microscopy-based biological assays

• Development of algorithms for quantitative microscopy

• Relationship between activation of the signaling pathway P2Y2-Go-Rac1, physical properties of the cell nucleus and the motility of glioma cells.

More information about Laboratory on the web

Selected publications:

 

Rybak  P.,  Hoang  A.,  Bujnowicz  L.,  Bernas T.,  Berniak  K.,  Zarebski  M.,  Darzynkiewicz  Z.,  Dobrucki  J.  (2016) Low level phosphorylation of histone H2AX on serine 139 (γH2AX) is not associated with DNA double-strand breaks. Oncotarget, doi: 10.18632/oncotarget.10411.

 

Campos Y., Qiu X., Gomero E., Wakefield R., Horner L., Brutkowski W., Young-Goo Han, Solecki D., Frase S., Bongiovanni A. & A. d’Azzo. (2016) Alix-mediated assembly of the actomyosin–tight junction polarity complex preserves epithelial polarity and epithelial barrier. Nat Commun, 7: 11876.

 

Sas-Nowosieska H., Bernaś T. (2016) Spatial relationship between chromosomal domains in diploid and autotetraploid Arabidopsis thaliana nuclei. Nucleus, 7(2): 216-31.

 

Bernaś T., Brutkowski W., Zarębski M., Dobrucki J. (2014) Spatial heterogeneity of dynamics of H1 linker histone. Eur Biophys J, 43 (6): 287-300.

 

L. Ramapathiran, Bernaś T., F. Walter, L. Williams, H. Düssmann, CG. Concannon and J.H. Prehn (2014) Single-cell imaging of the heat-shock response in colon cancer cells suggests that magnitude and length rather than time of onset determines resistance to apoptosis. J Cell Sci, 127: 609-619.