Laboratory of Molecular and Systemic Neuromorphology
Head: Grzegorz WILCZYŃSKI
Staff: Monika Malinowska, Joanna Dzwonek, Adriana Magalska, Maciej Gawlak (PhD student), Agnieszka Walczak (PhD student), Małgorzata Broszkiewicz (PhD student), Andrzej A. Szczepankiewicz (PhD student), Kamil Parobczak (PhD student), Anna Konopka (PhD student), Anna Skupień (PhD student), Paweł Rutkowski, Iwona Czaban, Matylda Babraj (student), Paweł Trzaskoma (student), Anna Krepa (student), Olga Kasprzak (student)
Our research focuses on the structural and functional plasticity of the nervous system, in both health and disease. We study phenomena occurring at various levels of organization, including anatomical sub-structures of the brain, the nervous tissue with all its cellular constituents, subcellular-, and macromolecular levels. Among multiple mechanisms contributing to synaptic plasticity we are focused on two phenomena, e.g. remodeling of synaptic extracellular matrix and adhesion molecules, and architectural changes of the neuronal cell nucleus associated with activity-dependent gene expression. We are also interested in advances of microscopy and tissue-visualization techniques.
Immunocytochemistry and in situ hybridization with confocal- or electron-microscopic detection. High resolution in situ zymography. Neuroanatomical methods. Molecular biology techniques.
1. Investigation of the activity-dependent interaction of the Bdnf gene with the nuclear lamina
2. Studies on the role of PML bodies in the adult brain
3. Studies on the ultrastructural remodeling of the interchromatin compartment of the neuronal cell nucleus upon seizures
4. Investigation of the role of Arc protein in the cell nucleus
5. Studies on immediate-early gene expression during spatial learning
6. Studies on the role of CD44, an adhesion and signaling receptor, in dendritic arborization and synaptic plasticity
7. Studies on the role of CD44 in astrocyte function and dysfunction
8. Structural and functional visualization of the brain using optical coherence tomography (in collaboration with Medical Physics group from Nicolaus Copernicus University in Torun).
Walczak A, Szczepankiewicz AA, Ruszczycki B, Magalska A, Zamlynska K, Dzwonek J, Wilczek E, Żybura-Broda K, Rylski M, Malinowska M, Dąbrowski M, Szczepińska T, Pawłowski K, Pyskaty M, Włodarczyk J, Szczerbal I, Świtoński M, Cremer M, Wilczyński GM. Novel Higher-Order Epigenetic Regulation of the Bdnf Gene upon Seizures. J Neurosci. 2013, 33:2507-2511
Yeghiazaryan M, Żybura-Broda K, Cabaj A, Włodarczyk J, Sławińska U, Rylski M,Wilczyński GM. Fine-structural distribution of MMP-2 and MMP-9 activities in the rat skeletal muscle upon training: a study by high-resolution in situ zymography. Histochem Cell Biol. 2012, 138:75-87.
Michaluk P, Wawrzyniak M, Alot P, Szczot M, Wyrembek P, Mercik K, Medvedev N,Wilczek E, De Roo M, Zuschratter W, Muller D, Wilczynski GM, Mozrzymas JW, Stewart MG, Kaczmarek L, Wlodarczyk J. Influence of matrix metalloproteinase MMP-9 on dendritic spine morphology. J Cell Sci. 2011, 124:3369-3380.
Wesierska M, Adamska I, Malinowska M. Retrosplenial cortex lesion affected segregation of spatial information in place avoidance task in the rat. Neurobiol Learn Mem; 91:41-49, 2009
Gorlewicz A, Włodarczyk J, Wilczek E, Gawlak M, Cabaj A, Majczyński H, Nestorowicz K, Herbik MA, Grieb P, Slawińska U, Kaczmarek L, Wilczyński GM. CD44 is expressed in non-myelinating Schwann cells of the adult rat, and may play a role in neurodegeneration-induced glial plasticity at the neuromuscular junction. Neurobiol Dis 2009; 34:245-258.
Gawlak M, Górkiewicz T, Gorlewicz A, Konopacki FA, Kaczmarek L, Wilczyński GM. High resolution in situ zymography reveals matrix metalloproteinase activity at glutamatergic synapses. Neuroscience; 2009; 158:167-176
Wilczyński GM, Konopacki FA, Wilczek E, Lasiecka Z, Gorlewicz A, Michaluk P, Wawrzyniak M, Malinowska M, Okulski P, Kołodziej LR, Konopka W, Duniec K, Mioduszewska B, Nikolaev E, Walczak A, Owczarek D, Górecki DC, Zuschratter W, Ottersen OP, and Kaczmarek L. Important role of matrix metalloproteinase 9 (MMP-9) in epileptogenesis. J Cell Biol 2008; 180: 1021-1035