Laboratory of Molecular Basis of Cell Motility

Head: Maria Jolanta RĘDOWICZ

Staff: Jolanta Nowak, Magdalena Bandyszewska (PhD student), Hanna Nieznańska, Krzysztof Nieznański, Iuliia Pavlyk (PhD student), Paweł Pomorski, Magdalena Sobczak, Dariusz Stępkowski, Małgorzata Suszek (PhD student), Emilia Wojtera, Tomasz Zajkowski (PhD student)

Research profile:

• involvement of unconventional myosins and actin-bind- ing proteins in cell migration and intracellular trafficking – studies on normal and transformed mammalian cells, and amoebae
• role of myosin VI in myogenesis and skeletal muscle functioning
• plasticity of the contractile apparatus from skeletal muscles
• role of prion protein-induced oligomerization of tubulin and phosphorylation of tau protein in the pathogenesis of prion diseases
• effects of arginine deprivation on actin cytoskeleton, cell migration and invasiveness
• formation and cytoskeletal modulation of the receptor- generated calcium signal in living cells
• regulation and mechanism of capacitative calcium signal
• role of calcium signaling in the regulation of cell motility

Methods:

• protein chemistry
• biochemistry
• fluorescence spectroscopy
• molecular and cell biology (micromanipulations, measurements of intracellular ions concentration, in vivo observations of cell motility and adhesion)
• fluorescence and electron microscopy

Current research activities:

• investigation of the involvement of class VI myosin in cell locomotion and intracellular trafficking
• analysis of the contractile apparatus in normal and atrophied muscles
• probing the interaction of prion protein with microtubular cytoskeleton
• fast calcium signal measurements
• imaging of receptor-induced calcium signal in primary culture neurons
• crosstalk between calcium and RhoA signaling pathways

Selected publications:

 

Nieznanska H, Dudek E, Zajkowski T, Szczesna E, Kasprzak AA, Nieznanski K. (2012) Prion protein impairs kinesin-driven transport. Biochem Biophys Res Commun 425:788-793. 

 

Karolczak J., Sobczak M., Majewski Ł., Yeghiazaryan M., Jakubiec-Puka A., Ehler E., Sławińska U., Wilczyński G., Rędowicz M.J. (2013) Myosin VI in skeletal muscle: its distribution in the sarcomere, neuromuscular junction and muscle nuclei. Histochem Cell Biol 139:873-885.

 

Nieznanski K, Surewicz K, Chen S, Nieznanska H, Surewicz WK. (2014) Interaction between prion protein and Aβ amyloid fibrils revisited. ACS Chem Neurosci 5:340-345.

 

J.P. Fichna, J. Karolczak, A. Potulska-Chromik, P. Miszta, M Berdyński, A. Sikorska, S. Filipek, M.J. Rędowicz, A.M. Kamińska, C. Żekanowski (2014) Desmin gene mutations associated with myofibrillar myopathies in the Polish population. PLOS One 9(12):e115470.

 

I Pavlyk, Y Rzhepetskyy, AK Jagielski, J. Drozak, A. Wasik, G. Pereverzieva, M. Olchowik, LA. Kunz-Schughart, O Stasyk, MJ Redowicz (2015) Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation. Amino Acids 47, 199-212.