Laboratory of Molecular Neurobiology

Head: Bożena Kamińska-Kaczmarek

Staff: Iwona Ciechomska, Michał Dąbrowski, Aleksandra Ellert-Miklaszewska, Paweł Gajdanowicz, Anna Gieryng, Beata Kaza, Marta Kocyk (SMM PhD student), Michał Kloss (PhD student), Katarzyna Konarzewska (PhD student), Izabela Krystkowiak, Dorota Kulesza (PhD student), Marta Maleszewska, Jakub Mieczkowski (PhD student), Dominika Pszczółkowska (PhD student), Piotr Przanowski (PhD student), Kavita Ramji (SMM PhD student), Małgorzata Sielska (PhD student), Justyna Ulańska-Poutanen (SMM PhD student), Paweł Wiśniewski, Bartosz Wylot, Małgorzata Zawadzka

Research profile:
We focus on identification and functional analysis of signalling pathways, transcriptional and epigenetic mechanisms controlling gene expression in microglia and tumour cells under disease-relevant conditions. Using chromatin immunoprecipitation, transcriptomics, next generation sequencing and bioinformatics we study functions of the key transcription factors: STATs, NFkB, Id and Myc, and iden- tify novel target genes and genetic networks. Genome wide transcription factor binding profiles and epigenetic modification database of human, rat and mouse genomes is developed. We develop RNAi or short interfering peptide- based approaches as potential therapeutic strategies to cure human diseases. Core facility activities: transcriptome anal- ysis, genome wide chromatin immunoprecipitation, next generation sequencing, data mining services for transcrip- tomics, genomics and proteomics, screening for inhibitors of signalling pathways and histone modifying enzymes.


Methods:
• cellular and animal models of neuroinflammation and tumour-host interactions
• chromatin immunoprecipitation
• transcriptome and methylome analyses, next generation sequencing
• production of cells expressing shRNA or recombinant proteins

Current research activities:

• identification of STAT dependent genes critical for the initiation of neuroinflammation and cancer
• studying a role of transcriptional and epigenetic changes in polarization of brain macrophage and immune micro- environment of gliomas
• development of RNAi – and recombinant interfering pep- tide-based molecules against signalling molecules driving pro-tumorigenic activation of brain macrophages
• isolation and molecular characterization of cancer stem cells

 

More information: www.kaminska-lab.pl


Selected publications:
Ciechomska I.A. Gabrusiewicz K., Szczepankiewicz A.A., Kamińska B. (2012) Endoplasmic reticulum stress triggers autophagy in malignant glioma cells undergoing cyclosporine A-induced cell death. Oncogene, doi: 10.1038/onc.2012.174.

Światek-Machado K., Mieczkowski J., Ellert-Miklaszewska A., Świerk P., Fokt I., Szymański S., Skora S., Szeja W., Grynkiewicz G., Lesyng B., Priebe W., Kamińska B. (2012) Novel small molecular inhibitors disrupt the JAK/STAT3 and FAK signaling pathways and exhibit a potent antitumor activity in glioma cells. Cancer Biology & Therapy, 13: 657-670.

Gabrusiewicz K., Ellert-Miklaszewska A., Lipko M., Sielska M., Frankowska M., Kamińska B. (2011) Characteristics of the alterna- tive phenotype of microglia/macrophages and its modulation in experimental gliomas. PLOS ONE, 6:e23902.

Kwiatkowska A., Kijewska M., Lipko M., Hibner U., Kamińska B. (2011) Down-regulation of Akt and FAK phosphorylation reduces invasion of glioblastoma cells by impairment of MT1-MMP shuttling to lamellipodia and down-regulates MMPs expression. Biochimica et Biophysica Acta, 1813: 655-667.

Tyburczy M.E., Kotulska K., Pokarowski P., Mieczkowski J., Kucharska J., Grajkowska W., Roszkowski M., Jóźwiak S., Kamińska B. (2010) Novel proteins regulated by mTOR in subependymal giant cell astrocytomas of Patients with Tuberous Sclerosis Complex and new therapeutic implications. American Journal of Pathology, 176: 1878-1890