LABORATORY OF PRECLINICAL STUDIES IN NEURODEGENERATIVE DISEASES

Head: Grażyna Niewiadomska

 

Degrees:

2008 DSc Habil, Nencki Institute of Experimental Biology, PAS

1988 PhD in Biology, Nencki Institute of Experimental Biology, PAS

1978 MSc in Biology, Department of Biology, University of Warsaw

 

Research trainings:

1999, 2002, 2003, 2006 Post-doctoral   training and fellowships, Biomedical Sciences, IMS, Aberdeen University, UK

1997 Post-doctoral training and fellowships, Department of Comparative Physiology, Etvos University, Hungary

1990-1991 Post-doctoral training and fellowships, Department of Neuroanatomy, Semelweiss University, Budapest, Hungary

 

Professional employments:

2010-present Associate Professor, Head of the Laboratory of Preclinical Studies in Neurodegenerative Diseases, Nencki Institute of Experimental Biology, PAS

2008-2010 Assistant Professor, Nencki Institute of Experimental Biology, PAS

1991-2008 Senior researcher, Nencki Institute of Experimental Biology, PAS

1984-1991 Researcher, Nencki Institute of Experimental Biology, PAS

1984-1984 Assistant, Nencki Institute of Experimental Biology, PAS

1982-1984 PhD student, Nencki Institute of Experimental Biology, PAS

1980-1982 PhD student, Mossakowski Medical Research Centre, PAS

 

Honors and fellowships:

2014 Jerzy Konorski Award of the Polish Neuroscience Society and the Committee of Neurological Sciences of the Polish Academy of Sciences for the Best Publication in Neurobiology.

2008 Silver Cross of Merit from the President of the Republic of Poland for the research on the role of hyperphosphorylation of tau protein in the dysfunction of microtubular transport

2007 present Member of the Scientific Steering Committee of WISTA LABORATORIES Ltd., University of Aberdeen

Staff: Teresa Cymbalak, Radosław Folcik, Anna Gąsiorowska, Ewelina Pałasz (PhD student), Marta Steczkowska, Adrianna Wysocka (PhD student), Maciej Zadrożny



Research profile:

Neurodegeneration  is  a  common  theme  of  many  nervous  system  diseases,  such as  Alzheimer’s  disease,  Parkinson’s  disease,  ALS,  head  trauma,  epilepsy  and  stroke. The occurrence of these devastating disorders is increasing rapidly in the ageing popula- tion and current treatments are inadequate. Our main research interest is to understand the mechanisms involved in neural ageing. Towards this end, we have tried to implement new experimental protocols and conduct longitudinal studies that can be used in neurodegen- erative disorders like Alzheimer’s disease and Parkinson’s disease. The work uses rats and transgenic mice and has centred on studies of the reversal of brain dysfunction induced by ageing. We attempt to link cellular and behavioral levels of the brain processes present during physiological and pathological ageing. Therefore, we develop quantitative approaches to the study of behaviour which offer to analyse the relationships between the elements of a given behavioural proxies. Currently the additional team’s main interest is the development of novel treatments, animal models and diagnostic procedures for the nervous system's diseases and assessment of toxicology, safety pharmacology, pharmacokinetics and complex behavioral effects of therapeutic compounds.




Current research activities:
 

• Assessment of the state and function of the brain cholinergic system in animal models of Alzheimer-type and frontotemporal dementia-type tauopathy

– behavioural, morphologic and biochemical evaluation of the cholinotrophic system in two transgenic mouse lines which develop mild (TgL1) and advanced (TgL66) tauopathy without amyloidopathy

– cholinergic anabolism and signalling

– the retrograde transport in basal forebrain cholinergic neurones and correlation of these changes with age-dependent structural reorganisation of the axonal cytoskeleton

– TrkA receptor expression and NGF-TrkA signalling

– cytoskeletal transport and post-translational modifications of microtubule

associated proteins during physiological ageing and neurodegenerative diseases in animal models

– profiling age-related cognitive impairments in spatial memory tasks.

• Counteraction Parkinson's disease by application of progressive training in humans and evaluation of factors which determine neuroprotective effect of this training using an animal model of Parkinson's disease

– effects of time of physical training initiation, its duration and intensity on the number and morphology of dopaminergic neurons in substantia nigra and in the ventral tagmental area, on the neurotrophic factors levels and brain tissue inflammation, and on motor function in mouse models of Parkinsonism.

• Development of novel treatments for neurodegenerative diseases: testing in animal models and assessment of drug safety and pharmacology.

 


Selected publications:

 

Niewiadomski W., Palasz E., Skupinska M., Zylinski M., Steczkowska M., Gasiorowska A., Niewiadomska G., Riedel G. (2016) TracMouse: A computer aided movement analysis script for the mouse inverted horizontal grid test. Sci Rep, 6: 39331.

 

Koss D.J., Robinson L., Mietelska-Porowska A., Gasiorowska A., Sepčić K., Turk T., Jaspars M., Niewiadomska G., Scott R.H., Platt B., Riedel G. (2015) Polymeric alkylpyridinium salts permit intracellular delivery of human Tau in rat hippocampal neurons: requirement of Tau phosphorylation for functional deficits. Cell Mol Life Sci, 72(23): 4613-4632.

 

Melis V., Zabke C., Stamer K., Magbagbeolu M., Schwab K., Marschall P., Veh R.W., Bachmann S., Deiana S., Moreau P.H., Davidson K., Harrington K.A., Rickard J.E., Horsley D., Garman R., Mazurkiewicz M., Niewiadomska G., Wischik C.M., Harrington C.R., Riedel G., Theuring F. (2015) Different pathways of molecular pathophysiology underlie cognitive and motor tauopathy phenotypes in transgenic models for Alzheimer's disease and frontotemporal lobar degeneration. Cell Mol Life Sci, 72(11): 2199-2222.

 

Mietelska-Porowska A., Wasik U., Goras M., Filipek A., Niewiadomska G. (2014) Tau protein modifications and interactions: their role in function and dysfunction. Int J Mol Sci, 15(3): 4671-4713.

 

Wasik U., Schneider G., Mietelska-Porowska A., Mazurkiewicz M., Fabczak H., Weis S., Zabke C., Harrington C.R., Filipek A., Niewiadomska G. (2013) Calcyclin binding protein and Siah-1 interacting protein in Alzheimer's disease pathology: neuronal localization and possible function. Neurobiol Aging, 34(5): 1380-1388.