Laboratory of Signal Transduction

Head: Tomasz Wilanowski

Staff: Aleksandra Glowacka (MSc student), Grzegorz Kotarba (postdoc), Ewa Krzywinska (postdoc), Magdalena Pawlak (PhD graduate), Agnieszka Taracha (PhD student), Marek Zorawski (Fulbright scholar)


Research profile:


Signal transduction in mammalian cells; role of the Grainyhead-like (GRHL) transcription factors in signal transduction; regulation of gene expression by the GRHL transcription factors; regulation of the activity of GRHL proteins; analysis of phenotypes of mouse strains carrying mutations in the Grhl genes; mechanisms of GRHL function in health and disease.


Methods:


• PCR
• quantitative PCR
• DNA sequencing
• histology
• immunohistochemistry
• immunofluorescence
• Western blotting
• chromatin immunoprecipitation
• electrophoretic mobility shift assays
• transfection studies
• phenotypic analyses


Current research activities:


• functions of the GRHL1 gene in human disease
• analysis of the phenotype of Grhl1 mutant mice
• mechanisms of regulation of the activity of GRHL1 protein
• regulation of gene expression by the GRHL1 transcription factor
• role of the GRHL factors in cancer


Selected publications:

 

Cangkrama M, Darido C, Georgy SR, Partridge D, Auden A, Srivastava S, Wilanowski T, Jane SM (2016) Two ancient gene families are critical for maintenance of the mammalian skin barrier in postnatal life. Journal of Investigative Dermatology 136: 1438-1448

Mlacki M, Kikulska A, Krzywinska E, Pawlak M, Wilanowski T (2015) Recent discoveries concerning the involvement of transcription factors from the Grainyhead-like family in cancer. Experimental Biology and Medicine 240: 1396-1401

Mlacki M, Darido C, Jane SM, Wilanowski T (2014) Loss of Grainy head-like 1 is associated with disruption of the epidermal barrier and squamous cell carcinoma of the skin. PLOS ONE 9: e89247

Darido C, Georgy SR, Wilanowski T, Dworkin S, Auden A, Zhao Q, Rank G, Srivastava S, Finlay MJ, Papenfuss AT, Pandolfi PP, Pearson RB, Jane SM (2011) Targeting of the tumor suppressor GRHL3 by a miR-21-dependent proto-oncogenic network results in PTEN loss and tumorigenesis. Cancer Cell 20: 635-648

Caddy J, Wilanowski T, Darido C, Dworkin S, Ting SB, Zhao Q, Rank G, Auden A, Srivastava S, Papenfuss TA, Murdoch JN, Humbert PO, Parekh V, Boulos N, Weber T, Zuo J, Cunningham JM, Jane SM (2010) Epidermal wound repair is regulated by the planar cell polarity signaling pathway. Developmental Cell 19: 138-147