Laboratory of Signal Transduction

Head: Tomasz Wilanowski

Staff: Aleksandra Glowacka (MSc student), Grzegorz Kotarba (postdoc), Ewa Krzywinska (postdoc), Magdalena Pawlak (PhD graduate), Agnieszka Taracha (PhD student), Marek Zorawski (Fulbright scholar)


Research profile:


Signal transduction in mammalian cells; role of the Grainyhead-like (GRHL) transcription factors in signal transduction; regulation of gene expression by the GRHL transcription factors; regulation of the activity of GRHL proteins; analysis of phenotypes of mouse strains carrying mutations in the Grhl genes; mechanisms of GRHL function in health and disease.


Methods:


• PCR
• quantitative PCR
• DNA sequencing
• histology
• immunohistochemistry
• immunofluorescence
• Western blotting
• chromatin immunoprecipitation
• electrophoretic mobility shift assays
• transfection studies
• phenotypic analyses


Current research activities:


• functions of the GRHL1 gene in human disease
• analysis of the phenotype of Grhl1 mutant mice
• mechanisms of regulation of the activity of GRHL1 protein
• regulation of gene expression by the GRHL1 transcription factor
• role of the GRHL factors in cancer


Selected publications:

 

Kikulska A, Rausch T, Krzywinska E, Pawlak M, Wilczynski B, Benes V, Rutkowski P, Wilanowski T (2018) Coordinated expression and genetic polymorphisms in Grainyhead-like genes in human non-melanoma skin cancers. BMC Cancer 18: 23
 

Pawlak M, Kikulska A, Wrzesinski T, Rausch T, Kwias Z, Wilczynski B, Benes V, Wesoly J, Wilanowski T (2017) Potential protective role of Grainyhead-like genes in the development of clear cell renal cell carcinoma. Molecular Carcinogenesis 56: 2414-2423
 

Cangkrama M, Darido C, Georgy SR, Partridge D, Auden A, Srivastava S, Wilanowski T, Jane SM (2016) Two ancient gene families are critical for maintenance of the mammalian skin barrier in postnatal life. Journal of Investigative Dermatology 136: 1438-1448
 

Mlacki M, Darido C, Jane SM, Wilanowski T (2014) Loss of Grainy head-like 1 is associated with disruption of the epidermal barrier and squamous cell carcinoma of the skin. PLoS One 9: e89247
 

Darido C, Georgy SR, Wilanowski T, Dworkin S, Auden A, Zhao Q, Rank G, Srivastava S, Finlay MJ, Papenfuss AT, Pandolfi PP, Pearson RB, Jane SM (2011) Targeting of the tumor suppressor GRHL3 by a miR-21-dependent proto-oncogenic network results in PTEN loss and tumorigenesis. Cancer Cell 20: 635-648