LABORATORY OF TRANSPORT THROUGH BIOMEMBRANES

Head: Katarzyna Nałęcz

 

Degrees:

2001 Professor of Biological Sciences, nomination by the President of the Republic of Poland, Nencki Institute of Experimental Biology, PAS
1993 DSc Habil, specialized in biochemistry, Nencki Institute of Experimental Biology, PAS
1982 PhD in Biology (Diploma cum laudae), Nencki Institute of Experimental Biology, PAS
1976 MSc in Biology (Diploma cum laudae,) specialized in biochemistry, University of Warsaw

 

Research trainings:

1990-1992 Long-term stay, University of Berne, Switzerland
1982-1985 Long-term stay, University of Berne, Switzerland

 

Professional employments:

2002 Visiting professor, 5-month stay at the Université d’Artois, France
1998-present Head of the Laboratory of Transport through Biomembranes, Nencki Institute of Experimental Biology, PAS
2001- present Professor, Nencki Institute of Experimental Biology, PAS
1994 Associate professor, Nencki Institute of Experimental Biology, PAS
1985 Assistant professor, Nencki Institute of Experimental Biology, PAS
1982 Assistant, Nencki Institute of Experimental Biology, PAS

 

Honors and fellowships:

2003-2005 President of the Polish Neuroscience Society
1996 Polish Academy of Sciences awards for exceptional scientific achievements
1990 Polish Academy of Sciences awards for exceptional scientific achievements
1982 PhD Diploma cum laudae

Staff: Barbara Juraszek (PhD student), Dominika Jurkiewicz (PhD student), Vasylyna Kovalchuk (PhD student), Karolina Rogala (PhD student)

 

Reserach profile:

 

Studies on proteins transporting carnitine to the cell, in particular on organic cation/carnitine transporter – OCTN2 (SLC22A5) and amino acid transporter B(0,+) – ATB(0,+) (SLC6A14). The experiments have been focused on the mechanism of transporters trafficking to plasma membrane and the role of other proteins in this process. The main goal is to establish the role of transporters domains in interaction with other protein. The role of OCTN2 and ATB(0,+) in cancer cells is also investigated.

 

Methods:

  • cell culture (primary culture of brain cells and cells transfected with vectors containing the studied genes)
  • preparing vectors with mutated genes coding studied transporters
  • transport measurements
  • immunocytochemistry
  • quantitative estimation of gene expression
  • proteomics of hydrophobic proteins

 

Current research activities:

  • studies on the mechanism of ATB(0,+) exit from endoplasmic reticulum
  • studies on interaction of OCTN2 with PDZ-domain containing proteins
  • studies on OCTN2 proteome and transporter interaction with PP2A phosphatase
  • studies on ATB(0,+) in cancer cells

 

Selected publications:

 

Jurkiewicz D., Michalec K., Skowronek K., Nałęcz K.A. (2017) Tigh junction protein ZO-1 controls organic cation/carnitine transporter OCTN2 (SLC22A5) in a protein kinase C-dependent way, BBA-Mol Cell Res, 1864: 797-805.

 

Nałęcz K.A. (2017) Solute carriers in the blood-brain barrier: Safety in abundance, Neurochem Res, 42(3): 795-809.

 

Juraszek B., Nałęcz K.A. (2016) Protein phosphatase PP2A – a novel interacting partner of carnitine transporter OCTN2 (SLC22A5) in rat astrocytes, J Neurochem, 139, 537-551.

 

Michalec K., Mysiorek C., Kuntz M., Bérézowski V., Szczepankiewicz A.A., Wilczyński G., Cecchelli R., Nałęcz K.A. (2014) Protein kinase C restricts transport of carnitine by amino acid transporter ATB0,+ apically localized in the blood-brain barrier, Archiv Biochem Biophys, 544: 28-35.

 

Czeredys M.., Samluk Ł., Michalec K., Tułodziecka K., Skowronek K., Nałęcz K.A. (2013) Caveolin-1 – a novel interacting partner of Organic Cation/carnitine Transporter (Octn2): Effect of protein kinase C on this interaction in rat astrocytes, PLoS ONE, 8: e82105.